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Abstract #2394

Subcellular Distribution of Mn in Neurons Assessed by Synchrotron X-Ray Microprobe

Alexia Daoust1,2, Sylvain Bohic1,3, Emmanuel Luc Barbier1,2

1INSERM U836, Grenoble, France; 2Grenoble Institut des Neurosciences, Universit Joseph Fourier, Grenoble, France; 3European Synchrotron Radiation Facility (ESRF), Grenoble, France

In MEMRI protocols, the cellular distribution and the transport mechanisms remains still unclear. To obtain further insights, we used synchrotron X-ray microprobe to map the sub-cellular distribution of Mn, P, and Fe in N2A cells (neuron-like) and in hippocampal neurons. For control and non-toxic conditions, Mn was distributed in the perinuclear region for N2A and in the nucleus for hippocampal neurons. Exposing these cells to a toxic dose of Mn alters the sub-cellular distribution of Fe which is redistributed towards Mn-rich regions.