Claire Wary1,2, Thibaud Naulet1,2, Jean-Laurent Thibaud1,3, Aurlien Monnet1,2, Stephane Blot4, Pierre G. Carlier1,5
1NMR Laboratory, Institute of Myology, Paris, France; 2IdM NMR Laboratory, CEA, IBM, MIRCen, Paris, France; 3UPR of Neurobiology, Ecole Nationale Veterinaire d'Alfort , Maisons Alfort, France; 4UPR of Neurobiology, Ecole Nationale Veterinaire d'Alfort, Maisons Alfort, France; 5IdM NMR Laboratory, CEA, IBM, MIRCen, Paris, France
We have identified a resonance, 0.3 ppm upfield from cytosolic Pi (Pia), which appears to be a second, more alkaline, Pi resonance (Pib) in muscle of dystrophic dogs (GRMD). Interestingly, Pib/Pia decreased with disease progression, and pHb was correlated to pHa Chemical exchange by inverse hydrolysis of ATP was measured by a combination of magnetisation transfer measurements and T1 with magnetisation transfer, in an attempt to distinguish and chacterize the two components. The T1 of Pia and Pib were equivalent, but chemical exchange of both compartments were very reduced in GRMD dogs compared to controls.