Abstract #3443

Novel Approach for the Assessment of the Bioavailability of Exogenous Phosphate by In Vivo Dynamic 17O & 31P MRS & MRI

Gheorghe D. Mateescu^1,2, Chris A. Flask^1,3, Jeffrey L. Duerk^1,3

¹Radiology, Case Western Reserve University, Cleveland, OH, United States; ²Chemistry, Case Western Reserve University, Cleveland, OH, United States; ³Biomedical Engineering, Case Western Reserve University, Cleveland, OH, United States

This communication presents a novel application of in vivo dynamic ¹⁷O and ³¹P MRS and MRI for the assessment of the bioavailability of exogenous phosphate (dietary or pharmaceutical). The method consists of administration of ¹⁷O-enriched phosphate to mice, followed by dynamic measurement of the ¹⁷O label transfer to body water, that results from the hydrolytic reactions of phosphate metabolism. Alternately, an efficient measurement could be done in ³¹P MR with ¹⁷O decoupling. This new approach may become important in relating phosphate homeostasis defects to metabolic or other diseases.