Siranush Anna Sargsyan1, Kendra M. Hasebroock, Brandon Renner, Brian Larsen2, Conrad Stoldt2, V. Michael Holers, Joshua M. Thurman, Natalie Serkova
1Medicine, University of
Colorado Denver, Aurora, CO,
Lupus nephritis is characterized by immune-complex deposition as well as complement C3 activation within the glomeruli of the kidney. Percutaneous renal biopsy is the gold standard for monitoring disease activity, yet less invasive methods of obtaining biopsy information are needed. We have developed a non-invasive method for detecting tissue-bound iC3b/C3d using MRI and superparamagnetic iron oxide (SPIO) nanoparticles linked to the iC3b/C3d binding region of complement receptor type 2 (CR2). We found that CR2-targeted SPIO nanoparticles caused a reduction in the T2-relaxation time in nephritic kidneys corresponding to the peak glomerular iC3b deposition and disease severity.