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Abstract #3706

In Vivo Quantification of Particle Based & Gene Based MRI Reporters in the Rodent Brain

Janaki Raman Rangarajan1, Greetje Vande Velde2, Caroline Guglielmetti3, Ruth Vreys3, Marleen Verhoye3, Tom Dresselaers2, Annemie Van Der Linden3, Uwe Himmelreich2, Frederik Maes1

1Medical Image Computing - ESAT/PSI, K.U. Leuven, Leuven, Belgium; 2Biomedical NMR Unit, K.U. Leuven, Belgium; 3Bio-Imaging Lab, University of Antwerp, Belgium

Iron-oxide based particles or MRI reporter genes (e.g. ferritin) can be used for labeling and visualization of endogenous stem cells in the rodent brain. While the quantification of hypo-intense contrast induced by these MRI reporters using parametric T2-maps is often constrained by their low resolution and are sensitive to B1 inhomogeneity, we have developed an image analysis pipeline for regional quantification from 3D T2*-weighted MRI. We demonstrate its potential for in vivo assessment of both iron-oxide particle based and gene based MRI reporters, which creates new opportunities for large scale in vivo imaging studies, including longitudinal follow-up within the same animal.