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Abstract #3974

MRI Assessment of the Effect of Different Resuscitation Fluids on Cerebral Blood Flow & Edema Following Experimental Traumatic Brain Injury and Hemorrhagic Shock in Mice

Lesley M. Foley1, T. Kevin Hitchens1,2, John A. Melick3, Nancy T. Ho2, Tusey C. Tam2, Chien Ho1,2, Patrick M. Kochanek3,4

1Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, PA, United States; 2Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, United States; 3Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States; 4Departments of Critical Care Medicine, Pediatrics & Anesthesiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States


Using MRI we assessed brain and pulmonary edema and cerebral blood flow (CBF) after resuscitation, from TBI+HS using two conventional solutions, namely the crystalloid Lactated Ringers (LR), the colloid hextend (Hex), and an octomeric recombinant hemoglobin (rHb). MRI assessment of edema using 1/T1 seemed to provide a more sensitive measurement than wet weight/dry weight values. Recombinant hemoglobin whilst increasing CBF initially also produces slightly more edema than either LR or Hex. One possibility is that rapid restoration of CBF in the damaged brain with rHb results in perfusion of severely damage tissue and edema.

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