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Abstract #0023

19F/1H MRI of Brain Inflammation in Experimental Autoimmune Encephalomyelitis

Helmar Waiczies1, 2, Stefano Lepore1, 2, Jason M. Millward3, 4, Bettina Purfrst5, Thoralf Niendorf, 23, Sonia Waiczies1, 2

1Ultrahigh Field Imaging in Neuroinflammation, Experimental and Clinical Research Center (ECRC), a cooperation of the Charit Medical Faculty and the Max Delbrck Center for Molecular Medicine, Berlin, Deutschland, Germany; 2Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck-Center for Molecular Medicine, Berlin, Deutschland, Germany; 3Experimental and Clinical Research Center (ECRC), Charit University Medicine Campus Berlin Buch, Berlin, Deutschland, Germany; 4Experimental Neuroimmunology, Charit Universittsmedizin Berlin, , Berlin, Germany; 5Electron Microscopy, Max-Delbrueck-Center for Molecular Medicine, Berlin, Germany

In the present study we employed an animal model of MS, the Experimental Autoimmune Encephalomyelitis (EAE) to explore the in vivo uptake of fluorine (19F) nanoparticles by inflammatory cells during encephalomyleitis. Using a 32-leg 1H/19F birdcage coil dedicated for mouse head imaging, we detected and quantified 19F nanoparticles (containing perfluoro-15-crown-5-ether) taken up and transported by macrophages within the cerebellum following intravenous application. The application of 19F nanoparticles for imaging immune cells in conditions such as encephalomyelitis is an emerging field that will be ideal to study the kinetics of immune cell localization during the development of inflammation.