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Abstract #0060

In Vivo Hyperpolarized 13C-MRS Shows Abnormal Cardiac Metabolism in the PPARα Knockout Mouse

MAGNA25Michael Dodd1, 2, Rosalind Bray1, Vicky Ball1, Mark Cole1, Kieran Clarke1, Damian Tyler1

1Department of Physiology, Anatomy and Genetics, Oxford University, Oxford, OXON, United Kingdom; 2Department of Cardiovascular Medicine, Oxford University, Oxford, OXON, United Kingdom


Peroxisome proliferator-activated receptor-α (PPARα)plays an important role in the regulation of fatty acid oxidation. The PPARα knockout mouse (PPARα-KO) has been developed to investigate the role of PPARα in cardiac metabolism and disease. This work aimed to assess the in vivo metabolic phenotype of PPARα-KO mice using hyperpolarized 13C-MRS. During the fed state, PPARα-KO mice had significantly elevated pyruvate dehydrogenase (PDH) flux compared to controls. During fasting there was a similar reduction in PDH flux in both control and PPARα-KO mice, indicating that this alteration is PPARα independent and is a response to increased fatty acid supply and utilization.

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