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Abstract #0060

In Vivo Hyperpolarized 13C-MRS Shows Abnormal Cardiac Metabolism in the PPARĪ± Knockout Mouse

MAGNA25Michael Dodd1, 2, Rosalind Bray1, Vicky Ball1, Mark Cole1, Kieran Clarke1, Damian Tyler1

1Department of Physiology, Anatomy and Genetics, Oxford University, Oxford, OXON, United Kingdom; 2Department of Cardiovascular Medicine, Oxford University, Oxford, OXON, United Kingdom

Peroxisome proliferator-activated receptor-α (PPARα)plays an important role in the regulation of fatty acid oxidation. The PPARα knockout mouse (PPARα-KO) has been developed to investigate the role of PPARα in cardiac metabolism and disease. This work aimed to assess the in vivo metabolic phenotype of PPARα-KO mice using hyperpolarized 13C-MRS. During the fed state, PPARα-KO mice had significantly elevated pyruvate dehydrogenase (PDH) flux compared to controls. During fasting there was a similar reduction in PDH flux in both control and PPARα-KO mice, indicating that this alteration is PPARα independent and is a response to increased fatty acid supply and utilization.