Eugene Kim1, Jana Cebulla2, B. Douglas Ward3, Kevin Rhie2, Jiangyang Zhang2, Arvind P. Pathak2, 4
1The Whitaker Biomedical Engineering Institute, Johns Hopkins University, Baltimore, MD, United States; 2The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University; 3Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, United States; 4The Johns Hopkins University in vivo Cellular and Molecular Imaging Center Program
There is a crucial need for noninvasive biomarkers of breast cancer angiogenesis to evaluate the efficacy of new anti-angiogenic therapies in vivo. Here, we validated in vivo steady-state susceptibility contrast (SSC)-MRI biomarkers of angiogenesis in an orthotopic human breast cancer model against the 3D vascular morphology obtained from high-resolution micro-computed tomography (CT). Based on cross-validation analysis, the simple parameters that only require ΔR2 and ΔR2* measurements were better predictors of their CT analogs than were the complex parameters that require additional measurements. Thus, a stand-alone SSC-MRI experiment provides promising candidates for noninvasive, in vivo biomarkers of breast cancer angiogenesis.