Sarah Woods1, Myriam Marianne Chaumeil1, Alessia Lodi1, Robert M. Danforth1, Aaron Robinson2, Joanna J. Philips2, Sabrina M. Ronen1
1Radiology, University of California, San Francisco, San Francisco, CA, United States; 2Neurological Surgery, University of California, San Francisco, San Francisco, CA, United States
Our goal was to assess the MRS-detectable metabolic changes associated with mutation of the isocitrate dehydrogenase (IDH) gene in two isogenic glioblastoma cell lines: U87IDHmutant and U87IDHwild-type. 1H MRS of extracts confirmed the presence of 2HG and detected several metabolic changes including elevated phosphocholine and elevated lactate in U87IDHmutant compared to U87IDHwt. The pyruvate-to-lactate conversion as measured by hyperpolarized 13C MRS was significantly lower in U87IDHmutant cells likely due to a drop in lactate dehydrogenase activity. Our studies inform on the metabolic consequence of the IDH mutation and identify biomarkers of the mutation.
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