Mapping of Oxygen by Imaging Lipids Relaxation Enhancement (MOBILE) in Experimental Tumor Models: Comparison with R2*, R1 H2O, and OxyLite Fiber Optic Probes.

Benedicte F. Jordan¹, Julie Magat¹, Elif Ozel¹, Florence Colliez¹, Anne-Catherine Fruytier¹, Valerie Marchand¹, Lionel Mignion¹, Caroline Bouzin², Olivier Feron², Bernard Gallez¹

There is a critical need for methods able to monitor dynamically and noninvasively tumor oxygenation. The purpose of the current work was to compare the MOBILE technique, a method developed to map variations in oxygenation based on the changes in the relaxation properties of the tissue lipids by exploiting the higher solubility property of oxygen in lipids than in water, with R2*, R1 H2O, and simultaneous quantitative oxygen measurements using fluorescence quenching fiber optic probes. Changes in tumor oxygenation were induced by an hyperoxic breathing challenge in order to determine correlations between the response assessed using each technique.