Glioma Cells Overexpressing Isocitrate Dehydrogenase 1 (IDH1) Mutation Produce 2-Hydroxy Glutaric Acid Detectable in vivo

Jelena Lazovic¹, Horacio Soto, David Piccioni, Arthur Chou, Sichen Li, Robert Prins, Linda Liau, Timothy Cloughesy, Albert Lai, Whitney Pope

The presence of isocitrate dehydrogenase 1 (IDH1) R132 mutation in gliomas including secondary glioblastoma is associated with more favorable outcome and increased overall survival compared to patients with functional IDH1. The aim of this study was to investigate metabolic changes and tumor growth rates in U87 glioma cells transformed to express mutated IDH1-R132. We found IDH1-R132 overexpression to be associated with an increase in tumor growth rate. Metabolic changes associated with the IDH1-R132 mutation included elevated levels of 2-hydroxyglutaric acid and reduced glutamate levels.