Cholesterol Detection in Adipose Tissue by Natural Abundance in vivo 13C MRS at 7T

Xing Chen¹, Peter Boesiger¹, Anke Henning¹

Adipose tissue is a major site of cholesterol storage. A variety of nutritional and metabolic alteration may influence the adipose tissue cholesterol level which has been studied in rats by chemical analysis. Localized natural abundance 13C MRS has been shown to be a powerful noninvasive technique for the study of lipids in vivo and has been applied to study the major classes of fatty acids. In this work, detection of cholesterol is reported for the first time noninvasively in human calf adipose tissue by 13C MRS at 7T, with J-refocused 1H PRESS localized DEPT sequence combined with broadband decoupling.