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Abstract #1773

Investigation on Neuroprotective Role of Caffeine Against MPTP Induced Neurotoxicity in Mice Using 13C NMR Spectroscopy

Puneet Bagga1, Suresh Kumar M1, Anant Bahadur Patel1

1NMR Microimaging and Spectroscopy, Centre for Cellular and Molecular Biology, Hyderabad, Andhra Pradesh, India

MPTP is a well established neurotoxin which causes selective degeneration of dopaminergic neurons in substantia nigra leading to dopamine loss and hence PD. Neuroprotective effects of caffeine, a MAO-B inhibitor against MPTP were investigated using infusion of [1,6-13C2]glucose and 1H-[13C]-NMR spectroscopy in adult male C57BL6 mice. Treatment with MPTP led to loss of glutamatergic and GABAergic activity in cortex, striatum, thalamus-hypothalamus and olfactory bulb. Pretreatment with caffeine led to recovery of glutamatergic and GABAergic activity in cortex and olfactory bulb, but the same in striatum and thalamus-hypothalamus was only partially recovered, suggesting partial neuroprotection conferred by caffeine against MPTP neurotoxicity.