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Abstract #1782

Enzymatic Conversion of [1-13C]pyruvate to [1-13C]lactate in Glioma Rat Model Evaluated by 11.7T NMR Correlated with DCE-MRI at 3T.

Jin Seo1, Hyun Jin Park1, Youn-Ki Nam2, Young Han Lee1, Ho-Taek Song1, Jin-Suck Suh1

1College of Medicine, Yonsei University, Seoul, Korea, Republic of; 2Agilent Technologies Korea Ltd., Seoul, Korea, Republic of

Among the various metabolites involved in the glucose metabolism of the tumor, pyruvate and lactate are the highlighted metabolites in the up-regulated glucose metabolism of malignant neoplasm. The purpose of this study is to determine the enzymatic conversion of [1-13C]pyruvate to [1-13C]lactate with exclusion of magnetization transfer effect from the hyperpolarized 13C. Enzymatic conversion ratio of [1-13C]lactate/pyruvate in glioma and normal brain were evaluated ex-vivo by using 11.7T NMR. DCE-MRI elucidated the vascular permeability characteristics of highly metabolic glioma. In-vitro cellular LDH assay of C6 glioma cells proved enzyme activity. The conversion ratio and quantitative DEC-MRI parameter Kep were correlated.