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Abstract #1787

Altered Hepatic ATP Storage and Creatine Biosynthesis in Transgenic Mouse Liver Expressing Creatine Kinase

Kamaiah Jayalakshmi1, 2, Min-Hui Cui1, 3, Wei Zhang2, Laibin Liu2, Craig A. Branch1, 3, Chandan Guha2

1Gruss Magnetic Resonance Research Center, Albert Einstein College of Medicine, Bronx, NY, United States; 2Radiation Oncology, Albert Einstein College of Medicine; 3Radiology, Albert Einstein College of Medicine, Bronx, NY, United States

31P MRSI has been employed to estimate hepatic phosphagen and ATP concentrations in transgenic mice expressing creatine kinase (CK-Tg) in liver with administration of cyclocreatine. A marked amount of phosphocyclocreatine was stored in liver of CK-Tg mouse with administration of cyclocreatine. In contrast, hepatic ATP concentrations decreased. These were accompanied by decreased serum creatine levels and increased urinary creatine levels. The significant amount of phosphocyclocreatine stored in CK-Tg mouse liver may serve as a false feedback corepressor of creatine biosynthesis since liver is usually lack of phosphagen. Abundant phosphocyclocreatine, a poor creatine kinase substrate, may also impede ATP production.