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Abstract #2975

Quantitative 1H HR-MAS Using LC Model Shows Glutamate, Choline, Glycerophosphocholine, and Glucose as Biomarkers of Prostate Cancer

Alan Wright1, May-Britt Tessem2, Helena Bertilsson3, 4, Maria T. Grinde2, Guro F. Giskedegrd2, Jostein Halgunset3, 5, Anders Angelsen4, Arend Heerschap

1Radiology, Radboud University Nijmegen Medical Centre, Nijmegen, Gelderland, Netherlands; 2Department of Circulation and Medical Imaging, NTNU, Trondheim, Norway; 3Department of Laboratory Medicine and Childrens and Womens Health, NTNU, Trondheim, Norway; 4Department of Urology, St Olavs Hospital, Trondheim University Hospital,, Trondheim, Norway; 5Department of Pathology and Medical Genetics, St Olavs Hospital, Trondheim University Hospital, Trondheim, Norway

1H HR-MAS MRS was performed on 131 cores taken from surgically resected prostates from patients with a cancer diagnosis. The cores were characterized with histopathology for the tumour and non-tumour content. LCModel was used with a novel basis set of model metabolites made from careful assignments of a subset of spectra and a formate standard solution for robust and automated quantification procedure. Glutamate, choline and glycerophosphocholine were all found to be positive markers for the presence of tumour tissue, while glucose was significantly reduced in samples containing mostly tumour. Citrate and spermine were found to negatively correlate with the percentage of stroma in benign tissue.