Abstract #4328

In Vivo Measurement of ALDH2 Activity in Rat Liver Ethanol Model Using Dynamic MRSI of Hyperpolarized [1-13C]-Pyruvate

Sonal Josan¹,², Tao Xu³, Yi-Fen Yen⁴, Ralph Hurd⁴, Julio Ferreira⁵, Che-Hong Chen⁵, Adolf Pfefferbaum¹,⁶, Dirk Mayer¹,², Daniel Spielm

¹Neuroscience Program, SRI International, Menlo Park, CA, United States; ²Radiology, Stanford University, Stanford, CA, United States; ³Electrical Engineering, Stanford University, Stanford, CA, United States; ⁴Applied Science Laboratory, GE Healthcare, Menlo Park, CA, United States; ⁵Chemical and Systems Biology, Stanford University, Stanford, CA, United States; ⁶Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, United States

Aldehyde dehydrogenase-2 (ALDH2) enzyme is important for eliminating toxic aldehydes that accumulate in several diseases. It is also used in ethanol metabolism in the liver, producing NADH in the process. This work investigates using hyperpolarized [1-¹³C]-pyruvate MRSI for in vivo measurement of ALDH2 activity. Two different doses of ALDH2 inhibitor disulfiram were used to reduce ALDH2 activity in two groups with another group acting as control. Using ethanol metabolism to modulate the NADH availability in rat liver, the resulting change in pyruvate-to-lactate conversion was measured with ¹³C MRSI and correlated with ALDH2 enzyme activity.