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Abstract #4373

Characterization of Lung Tumor Cell Lines by Amide Proton Transfer (APT) Imaging in In-Vitro System

Kim Kangasniemi1, Koji Sagiyama1, Boning Gao2, Chunxian Huang2, John Minna3, Masaya Takahashi1

1Advanced Imaging Research Center, UT Southwestern Medical Center, Dallas, TX, United States; 2Hamon Center for Therapeutic Oncology, Pharmacology, University of Texas Southwestern Medical Center, UT Southwestern Medical Center, Dallas, TX, United States; 3Hamon Center for Therapeutic Oncology, Internal Medicine, Pharmacology, UT Southwestern Medical Center, Dallas, TX, United States


The proton exchange between amide groups in mobile proteins/peptides and free water in two different human cancer cell lines and normal cell line were studied. Amide proton transfer (APT) imaging is one of the chemical exchange saturation transfer (CEST) imaging methods was utilized to differentiate between the tumors. A comparison in magnetization transfer between cell tumor lines showed a difference in APT signal, suggesting that tumor type characterization can be done by in-vivo APT imaging.

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