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Abstract #0160

A Bright Multi-Imaging Modality Gene Reporter

P. Stephen Patrick1, 2, Jayne Hammersley2, Louiza Loizou2, Mikko I. Kettunen1, Tiago B. Rodrigues1, De-En Hu2, Sui Seng Tee, 23, Robin Hesketh2, Scott K. Lyons1, Silvio Aime4, Sandra M. Fulton2, Kevin M. Brindle, 12

1Cancer Research UK, University of Cambridge, Cambridge, Cambridgeshire, United Kingdom; 2Department of Biochemistry, University of Cambridge, Cambridge, Cambridgeshire, United Kingdom; 3School of Medicine, University of Stanford, Palo Alto, CA, United States; 4Molecular Biotechnology Centre, University of Turin, Turin, Piedmont, Italy

A new reporter gene for MRI is described that gives rapid, intense, and reversible T1-weighted contrast enhancement. We have achieved this through the expression of Oatp1, which is a transporter for the clinically approved contrast agent gadoxetate. R1 was enhanced by over four fold in reporter expressing tissue 5 hours post intravenous gadoxetate injection, while R1 for control tissue was not significantly different than before injection. This system therefore has the