Abstract #0238

Two Component System for MR/optical Image-Guided Delivery and Cell Surface Targeting of HER2(+) Cancer Cells.

Sudath Hapuarachchige¹, Wenlian Zhu¹, Yoshinori Kato¹,², Dmitri Artemov¹,²

¹Division of Cancer Imaging Research, Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United States; ²Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, United States

Here, we have demonstrated MR/optical image-guided delivery of a two-component system based on modified azido-trastuzumab and drug containing albumin functionalized with strained promoted alkynes. These two components undergo multiple click reactions and assemble on cell surface enhancing the cellular internalization. This strategy was used for the efficient and synergistic delivery of biotherapeutic (trastuzumab) and chemotherapeutic (paclitaxel) as potential new strategy for treatment of trastuzumab resistant HER2/neu overexpressing tumors. Specific delivery of the components to BT-474 xenografts and increased cytotoxicity in vitro in HER2/neu expressing cancer cells is reported.