Myocardial ATP Turnover Rates in the in vivo Hearts with Post-Infarction Remodeling

Qiang Xiong¹, Pengyuan Zhang¹, Lei Ye¹, Jinfeng Tian¹, Cory Swingen¹, Albert Jang¹, J. Thomas Vanghan¹, Jianyi Zhang¹

A novel indirect magnetization saturation transfer (MST) approach is developed to allow measurement of ATP→Pi reaction rate without quantification of inorganic phosphate (Pi) levels, thus avoided the primary barrier for applications in in vivo heart. With the new approach, myocardial ATP→Pi reaction rate was measured in Normal and post-infarct hearts with or without stem cell therapy. The data suggested that the ATP→Pi rate is a sensitive bioenergetic index that is tightly correlated to cardiac workload as well as the severity of post-infarction left ventricular remodeling based on a swine model of post-infarction LV remodeling.