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Abstract #0299

Neurometabolic and Neurovascular Couplings Across Cortical Layers of Rat Brain

Peter Herman1, Basavaraju G. Sanganahalli1, Douglas L. Rothman1, 2, Hal Blumenfeld3, 4, Fahmeed Hyder1, 2

1Department of Diagnostic Radiology, Yale University, New Haven, CT, United States; 2Biomedical Engineering, Yale University, New Haven, CT, United States; 3Neurology, Yale University, New Haven, CT, United States; 4Neurobiology, Yale University, New Haven, CT, United States


CMRO2 changes were calculated with calibrated fMRI at 11.7T measurements of CBF, CBV, and BOLD responses across layers of the rat cortex. LFP and MUA measurements were compared to BOLD, CBV, CBF, and CMRO2 responses. Surprisingly BOLD and CBV responses varied considerably across layers and were uncoupled with layer-specific neural measures - LFP was strong in all cortical layers, whereas MUA was weakest in the superficial layers. CBF was strongly correlated with LFP, whereas CMRO2 was correlated with MUA. While these results lend experimental support for neurometabolic and neurovascular couplings across the cortex, CBF/LFP and CMRO2 /MUA have different spatial distributions.

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