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Abstract #0439

Quantitative MRI Reporter Gene Imaging of the Recruitment of Ferritin Over-Expressing Fibroblasts to the Vascular Niche of Solid Tumors

Moriel Vandsburger1, Marina Radoul1, Yoseph Addadi1, Senzeni Mpofu1, Batya Cohen1, Michal Neeman1

1Weizmann Institute of Science, Rehovot, None, Israel

We used over-expression of ferritin heavy chain (FHC) as an MRI reporter gene in CV-1 fibroblasts. The recruitment of FHC over-expressing fibroblasts to ovarian cancer tumors was quantitatively examined using bi-exponential modeling in order to determine the cell fraction of FHC over-expressing cells within a mixed cell population. In addition, mapping of fractional blood volume revealed preferential recruitment of FHC over-expressing fibroblasts to the vascular niche of growing tumors.