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Abstract #1446

Differential Play of Macrophages and T-Lymphocytes in Acute Allograft Cardiac Rejection: in vivo Cellular MRI Detection with a New Iron-Oxide Particle

Yijen Lin Wu1, Li Liu1, Qing Ye1, Brent D. Barbe1, T. Kevin Hitchens1, Chien Ho1, 2

1Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, PA, United States; 2Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, United States

Cellular MRI with iron-oxide based contrast agents offers the potential to track many cell types in vivo at high resolution. The newly modified ITRI-IOPC-NH2 particles with increased positive charge and terminal amino groups can label T-lymphocytes by simple co-incubation without eternal aids. Our results show that T-lymphocytes and macrophages exhibit different temporal appearance behavior in the rejecting cardiac allograft, indicating that they may play different roles in rejection.