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Abstract #1512

NMR, LIBS and FTIR Based Metabolomics of an Antidiabetic Herbal Formulation

Prashant Kumar Rai1, Rama Jayasundar1, Gaurav Sharma1, Ashok Kumar Pathak2, Somenath Ghatak1, Geeta Watal3, A K. Rai2

1Department of NMR, All India Institute of Medical Sciences, New Delhi, Delhi, India; 2Department of Physics, University of Allahabad, Allahabad, U P, India; 3Department of Chemistry, University of Allahabad, Allahabad, U P, India

This study reports a comprehensive metabolomic profiling of an antidiabetic (evaluated in vivo in rats) polyherbal formulation using NMR, FTIR and LIBS (Laser Induced Breakdown Spectroscopy). The proton spectrum showed primary and secondary metabolites, many of which were further validated using FTIR spectroscopy. LIBS spectrum revealed the presence of elements such as Na, K, H, Mg and Ca. The presence of primary and secondary metabolites in NMR and FTIR spectra, and that of Mg and Ca in LIBS spectrum indicate their possible roles in the antidiabetic activity of the formulation. Mg and Ca are known to regulate blood glucose level.