Abstract #1856

Pharmacokinetics and Biodistribution of Superparamagnetic Nanoparticles Using PEPR in the Mouse: An in vivo, in Vitro and Ex Vivo Validation.

Oliviero Gobbo¹,², Friedrich Wetterling³, Kieran Crosbie-Staunton², Peter Vaes⁴, Stephanie Teughels⁴, Farouk Markos⁵, Marek Radomski¹, Adriele Prina-Mello², Yuri Volkov²

¹School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin, Ireland; ²School of Medicine, Trinity College Dublin, Dublin, Ireland; ³School of Engineering, Trinity College Dublin, Dublin, Ireland; ⁴PEPRIC nv, Leuven, Belgium; ⁵Physiology, University College Cork, Cork, Ireland

The biodistribution of superparamagnetic iron oxide nanoparticles (SPIO) was evaluated in a mouse model using MRI measurements and a new analytical technique based on particle electron paramagnetic resonance (pEPR). Additionally, the pEPR results were also compared to inductively coupled plasma mass spectrometry (ICP-MS) iron analysis. Our results indicate that both techniques were equally sensitive. The iron measurements by pEPR in the organs were consistent with signal intensity changes recorded by MRI. Overall the data showed that this new technique is suitable for pharmacokinetic and biodistribution studies of SPIO.