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Abstract #1890

The Effect of Cryoprotection on the Use of PLGA Encapsulated Iron Oxide Nanoparticles for Magnetic Cell Labeling

Erik M. Shapiro1, Kevin S. Tang2

1Department of Radiology, Michigan State University, East Lansing, MI, United States; 2Department of Biomedical Engineering, Yale University, New Haven, CT, United States

The objective of this study was to identify a cryoprotection scheme to enhance redispersion of PLGA encapsulated iron oxide NPs in water and to study the effect this would have on magnetic cell labeling. We discovered that optimal percentage of dextrose for retaining initial particle characteristics was 1% with increasing percentages having little effect on average particle size and distribution and decreasing percentages having increasing degrees of aggregation. We found that for magnetic cell labeling, increasing dextrose cryoprotection decreased labeling efficiency, however all cryoprotectant percentages labeled cells robustly enough to enable single cell detection on MRI.