Myriam Marianne Chaumeil1, Joydeep Mukherjee2, Humsa Venkatesh1, Russell O. Pieper2, Sabrina M. Ronen1
1Radiology, University of California, San Francisco, San Francisco, CA, United States; 2Neurological Surgery, University of California, San Francisco, San Francisco, CA, United States
The M2 isoform of pyruvate kinase (PKM2) induces a reprogramming of energetic metabolism in cancer cells, leading to tumor growth and metastasis. Because PKM2 modulation is being investigated as a therapeutic approach, we questioned whether hyperpolarized (HP) 13C MRS of pyruvate could serve as a method to probe the PKM2 status. This study shows that knocking down PKM2 expression in glioblastoma cells leads to a significant decrease in HP lactate formation. HP 13C MRS of pyruvate could thus prove useful for evaluation of new PKM2-targeted therapies.