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Abstract #3859

On the Fate of MRI Gd-Complexes in Cells. Evidence for Extensive Degradation of Linear Complexes.

Eliana Gianolio1, Enza Di Gregorio1, Rachele Stefania1, Silvio Aime1

1Molecular Biotechnologies and Health Sciences, University of Torino, Torino, TO, Italy

The use of highly stable complexes has been an important guide-line in the design of Gd-based contrast agents (Gd-CA). Many pre-clinical studies have been reported addressing the use of Gd-CA in cellular labeling experiments. In this presentation we report, for the first time, results concerning the stability of Gd-CA upon their cellular entrapment. Mass spectrometry was used for assessing the amount of intact Gd-complex inside cells upon time. Insights into the intracellular degradative mechanisms have been gained by analyzing the relaxometric NMRD profiles of cells labeled with the Gd-CA.