Tiago B. Rodrigues1, Eva M. Serrao1, Brett W.C. Kennedy1, De-En Hu1, Kevin M. Brindle1, Mikko I. Kettunen1
1Biochemistry/CRUK, University of Cambridge, Cambridge, United Kingdom
Tumor cells frequently display high rates of aerobic glycolysis, resulting in increased lactate production and correlating with a poor prognosis and increased tumor aggressiveness and metatasis. We show here that hyperpolarized [U-2H, U-13C]glucose allows real-time monitoring of tumor glycolysis in vivo, through the production of hyperpolarized [U-13C]lactate, and that this flux is substantially reduced in murine EL4 lymphoma tumors by 24 h after etoposide treatment. Although the sensitivity of MR detection of hyperpolarized [U-2H, U-13C]glucose is much lower than PET detection of FDG, it has some significant potential advantages for detecting tumor treatment response.