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Abstract #4003

Evolution of the Hepatic Lipid Profile of the Adult Mouse - in Vivo and in Vitro 1H MRS Assessments at 14.1T

Ana Francisca Soares1, Hongxia Lei2, Rolf Gruetter3, 4

1LIFMET, EPFL, Lausanne, Vaud, Switzerland; 2Department of Radiology, University of Geneva, Geneva, Switzerland; 3LIFMET, cole Polytechnique Fdrale de Lausanne, Lausanne, Vaud, Switzerland; 4University of Lausanne, Lausanne, Vaud, Switzerland

The hepatic lipid content and composition were assessed in healthy mice throughout adulthood. It was found that aging and obesity contributed to increase the amount of lipids in the liver while decreasing the poly-unsaturation degree. The combination of in vivo 1H MRS assessments with in vitro measurements on tissues extracts illustrated the important contribution of membrane lipids to the total poly-unsaturation degree of the fatty acyl chains. Changes of the unsaturation profile of cytosolic lipids can be monitored by in vivo 1H MRS, which is of interest for the study of genetic and diet-induced mice models of metabolic diseases.