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Abstract #4238

Detection of Protein Accumulation by Amide Proton Transfer (APT) in the Spinal Cord of SOD1 Mice Using Exchange-Modulated PRESS

Marilena Rega1, Francisco Torrealdea1, Phillip Smethurst1, James Dick1, Anna Gray1, Linda Greensmith1, Katie Sidle1, Simon Walker-Samuel2, David L. Thomas1, Xavier Golay3

1Institute of Neurology, UCL, London, Greater London, United Kingdom; 2Centre for Advance Biomedical Imaging, UCL, London, Greater London, United Kingdom; 3Institute of Neurology, University College London, London, Greater London, United Kingdom

Amide proton transfer (APT) is able to produce contrast originating from endogenous cellular proteins and peptides. In this study we explore the possibility of using APT as a biomarker of Amyotrophic lateral sclerosis (ALS) through a SOD1 mutate mouse model that closely resembles the disease. Comparisons of the APT signal between healthy and SOD1 animals at the same age, as well as validation of the results with protein assays (ex-vivo) show a significant difference between the two groups at a pre-symptomatic stage of the disease. These results suggest that APT could potentially become an early biomarker for ALS.