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Abstract #1749

Pyruvate to lactate metabolism with age in normal mice measured by hyperpolarized 13C

Yiran Chen 1 , Robert Bok 1 , Subramanian Sukumar 1 , Xin Mu 1 , Ann Sheldon 2,3 , A James Barkovich 1 , Donna M Ferriero 2,3 , and Duan Xu 1,4

1 Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA, United States, 2 Department of Neurology, University of California San Francisco, CA, United States, 3 Department of Pediatrics, University of California San Francisco, CA, United States, 4 Joint UCSF/UC Berkeley Graduate Group in Bioengineering, CA, United States

In this study, we applied dynamic nuclear polarization (DNP) technique to investigate C1 labeled 13C pyruvate to lactate conversion across maturation in mice in vivo. Normal mice were scanned starting on postnatal day 18 and repeated every 10 days. Experiments were conducted on a 14.1T NMR spectrometer with real-time metabolic imaging simultaneously with injection of hyperpolarized pyruvate. We found that lactate level was significantly higher at younger ages, and stabilized around P30. The level of lactate was also independent to the polarization or pyruvate level.

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