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Abstract #2762

Investigation of Cell Membrane Transport and Compartmentalisation of Hyperpolarised Metabolites using a GdDO3A Relaxation Agent

Markus Durst 1,2 , Ulrich Koellisch 1,2 , Francesca Reineri 3 , Valeria Daniele 3 , Concetta Gringeri 4 , Annette Frank 4 , Marion I. Menzel 2 , Axel Haase 1 , Rolf F. Schulte 2 , and Silvio Aime 3

1 IMETUM, Technische Universitt Mnchen, Garching, Germany, 2 GE Global Research, Garching, Germany, 3 Department Chemistry I.F.M. and Molecular Imaging Center, University of Torino, Torino, Italy, 4 Klinikum rechts der Isar, Technische Universitt Mnchen, Mnchen, Germany

Dynamic nuclear polarisation has enabled the analysis of metabolic pathways in real-time using hyperpolarised substrates. The conversion of [1-13C]pyruvate to downstream metabolites such as lactate and alanine can provide an important tool for studying diseases with abnormal cellular metabolism such as cancer or ischemia. In this work, an extracellular relaxation agent (GdDO3A) was used to quasi-instantaneously quench the extracellular compartment of the metabolites. In combination with a substrate saturation sequence, transporter rates for the cell membrane transport of hyperpolarised metabolites could be quantified for the first time in vivo.

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