Abstract #2814
Development and evaluation of labeled islet cells using iron oxide nanoparticles and DNA hybridization
Ryusuke Nakai 1,2 , Narufumi Kitamura 1 , and Hiroo Iwata 1
1
Institute for Frontier Medical Sciences,
Kyoto University, Kyoto, Japan,
2
Kokoro
Research Center, Kyoto University, Kyoto, Japan
In this study, we developed a labeling method for islet
cells with superparamagnetic iron oxide particles
(SPIOs) based on DNA hybridization, which has been
successfully applied to cell surface modification. The
labeling efficiency of this method was evaluated by MRI.
The SPIO-labeled islet cells could be clearly detected
as dark spots by T2*-weighted MR image, whereas
non-labeled islet cells could not be detected. The
method is also likely to be applicable for use with
other cells, such as stem cells, and for labeling at
multiple sites by changing the DNA sequence.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Click here for more information on becoming a member.
