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Abstract #2886

Intracellular Redox State Quantification by 31P MRS measurement of NADH and NAD+ Content during Ischemia/Reperfusion in Perfused Rat Heart

Charlie Yi Wang 1 , Ya Chen 2 , and Xin Yu 1

1 Biomedical Engineering, Case Western Reserve University, Cleveland, OH, United States, 2 Case Western Reserve University, Cleveland, OH, United States

Estimation of intracellular redox state using measured NADH/NAD+ ratio through 31P spectroscopy is an attractive tool, as alternative methods for direct assessment are invasive in nature. In this study, we assess the ability of 31P MRS to quantify the NADH/NAD+ ratio during ischemia/reperfusion injury in perfused rat heart.

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