Combination of a 13 C cryoprobe with hyperpolarization allows real time observation of pyruvate carboxylation in the perfused mouse heart

Murine models of myocardial metabolism are a pervasive tool used by the cardiovascular research community. Development of methods for monitoring energy metabolism in the perfused mouse heart would augment the understanding of a variety of myocardial pathologies and dysfunctions. Here, hyperpolarized pyruvate is combined with a ¹³ C optimized cryogenic probe to produce an approximate sensitivity gain of 140,000x for carbon spectroscopy. The resulting spectra in the functioning heart allow pyruvate carboxylation to be monitored in real time, a pathway accepted to have a relative activity of about 5 % of that of pyruvate dehydrogenase.

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