Absolute quantitation of metabolites in human prostate cancer biopsies by HR-MAS 1 H NMR spectroscopy

Human prostate biopsies were analysed with HRMAS ¹ H NMR spectroscopy. Absolute metabolite concentrations of the following metabolites were estimated by a modified LC-Model basis set in benign, low grade and high grade prostate cancer samples: alanine, lactate, glutamine, glutamate, citrate, choline, phosphocholine (PC)+ glycerophosphocholine(GPC), creatine + phosphocreatine, taurine, myo-inositol and spermine . Alanine was significantly elevated in both low and high-grade prostate cancer biopsies; total choline(choline+PC+GPC) and glutamate were significantly higher in high-grade malignant prostate. None of the macromolecule (0.9ppm, 2.0ppm) and lipid signals (0.9ppm, 1.3ppm and 2.0ppm) showed any statistical differences between the benign and cancer samples

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