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Abstract #0151

Validation of orientation distribution functions in 3D using confocal microscopy

Kurt Schilling 1 , Yurui Gao 1 , Vaibhav Janve 1 , Iwona Stepniewska 2 , Prasanna Parvathaneni 3 , Hua Li 1 , Bennett A Landman 4 , and Adam W Anderson 1

1 VUIIS, Vanderbilt University, Nashville, TN, United States, 2 Psychology, Vanderbilt University, Nasvhille, United States, 3 Electrical Engineering, Vanderbilt University, TN, United States, 4 Electrical Engineering, Vanderbilt University, Nashville, TN, United States

Validation of fiber orientation and anisotropy estimates from diffusion MRI (dMRI) studies has so far been limited to 2D analysis of histological sections. Here, we use structure tensor analysis to extract the 3D histological fiber orientation distribution (FOD) from confocal z-stacks, which then serves as a gold standard for comparisons with various dMRI acquisition protocols and signal models. Our results show good agreement between histological and dMRI estimates of fiber orientation in voxels containing nearly parallel fibers. Further, we find that Q-ball and DSI analysis are also able to resolve crossing fibers with similar accuracy.

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