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Abstract #1931

Fluorine-19 labelling of stromal vascular fraction cells for clinical imaging applications

Laura C Rose 1 , Guan Wang 1 , Brooke M Helfer 2 , Charles F O'Hanlon 2 , Amnon Bar-Shir 1 , Dara L Kraitchman 1 , Ricardo L Rodriguez 3 , and Jeff WM Bulte 1

1 Johns Hopkins University, Baltimore, MD, United States, 2 Research & Development, Celsense Inc, PA, United States, 3 CosmeticSurg LLC, Luthersville, MD, United States

Labeling cells with perfluorinated carbons allows tracking with 19 F-MRI in vivo . As part of clinical trial NCT02035085 and FDA IND submission, we labeled adipose-derived stromal vascular fraction (SVF) cells and determined uptake in cell subtypes. The majority (87-92%) of CD31+ and CD34+ stem cells were labeled but only 47% of CD45+ cells, highlighting SVF cell heterogeneity. With a 3T clinical set-up and 19 F content of 10 12 atoms/cell, 2x10 6 cells were detected in a breast phantom 5mm below the surface. With 1x10 8 SVF cells for breast reconstruction, this sensitivity will be sufficient to track transplanted SVF cells in breast cancer survivors.

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