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Abstract #2765

Evaluation of MR Contrast in Cleared Tissue

Christoph Leuze 1 , Raju Tomer 2 , Qiyuan Tian 1 , Emily Ferenczi 2 , Dan Spielman 1 , Michael Zeineh 1 , Karl Deisseroth 2,3 , and Jennifer A McNab 1

1 Radiology, Stanford University, Stanford, CA, United States, 2 Bioengineering, Stanford University, Stanford, CA, United States, 3 Psychiatry and Behavioural Research, Stanford University, Stanford, CA, United States

CLARITY is a tissue clearing technique that uses hydrogel-embedding to maintain the structural integrity of the tissue and spatial organization of proteins, nuclei acids and other small molecules while using a detergent to remove the lipids that render the tissue optically opaque. It is expected that biomolecules with an NH2 group will bind to the hydrogel and therefore not be removed by the clearing process. MRI of cleared tissue samples can serve the dual purpose of evaluating the efficacy of the tissue clearing and as a means to learn how much the cleared components, such as lipids, contribute to various types of MRI contrast. Here we demonstrate MR images with a range of different contrast mechanisms in a cleared human brain tissue sample and a cleared, whole, mouse brain.

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