Abstract #3894
2HG Metabolic Profiling Analysis based on 13C-NMR Spectroscopy with Stable13C-labeled Isotope
Hyeon-Man Baek 1,2 , Youngjae Jeon 1 , Jooyun Kim 1 , and Mirim Bang 1
1
Center for MR Research, Korea Basic Science
Institute, Ochang, Chungbuk, Korea,
2
Department
of Bio-Analytical Science, University of Science &
Technology, Daejeon, Chungnam, Korea
The aim of this study was two-fold: first, to
investigate the metabolic profiling analysis based on
13C-NMR spectroscopy with stable 13C-labeled isotope,
and second, to demonstrate whether 2HG labeling from
[U-13C]glucose substrate feeding could be detected in
IDH1 or 2 mutated cells.The analysis of 1H- and 13C-NMR
spectra of the cell extracts showed a significant
increase in the concentration of the 2HG in IDH mutated
cells, but not in IDH wild type and mutant IDH cells
with low transfection efficiency.
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