Meeting Banner
Abstract #3894

2HG Metabolic Profiling Analysis based on 13C-NMR Spectroscopy with Stable13C-labeled Isotope

Hyeon-Man Baek 1,2 , Youngjae Jeon 1 , Jooyun Kim 1 , and Mirim Bang 1

1 Center for MR Research, Korea Basic Science Institute, Ochang, Chungbuk, Korea, 2 Department of Bio-Analytical Science, University of Science & Technology, Daejeon, Chungnam, Korea

The aim of this study was two-fold: first, to investigate the metabolic profiling analysis based on 13C-NMR spectroscopy with stable 13C-labeled isotope, and second, to demonstrate whether 2HG labeling from [U-13C]glucose substrate feeding could be detected in IDH1 or 2 mutated cells.The analysis of 1H- and 13C-NMR spectra of the cell extracts showed a significant increase in the concentration of the 2HG in IDH mutated cells, but not in IDH wild type and mutant IDH cells with low transfection efficiency.

This abstract and the presentation materials are available to members only; a login is required.

Join Here