Meeting Banner
Abstract #0483

Pharmacological MRI combined with DREADD-technology enables detection of induced brain activity in projections relevant for feeding behavior

Tessa J.M. Roelofs1,2, Geralda A.F. van Tilborg1, Mieneke C.M. Luijendijk2, Roger A.H. Adan2, and Rick M. Dijkhuizen1

1Biomedical MR Imaging and Spectroscopy Group, Center for Image Sciences, University Medical Center Utrecht, Utrecht, Netherlands, 2Translational Neurosciences, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, Netherlands

With the aim to develop a novel MRI-based approach for detection of activation in neuronal networks associated with feeding behavior in a rat model, we evaluated the potential of pharmacological MRI (phMRI) to detect DREADD (Designer Receptor Exclusively Activated by Designer Drug)-evoked neuronal activity. BOLD phMRI was conducted under 1.5% isoflurane anesthesia at 9.4T. Pharmacological activation induced a significant BOLD response in DREADD-targeted areas, which was confirmed by cFos-based immunohistochemistry of neuronal activation. Our study shows that phMRI allows detection of specific DREADD-evoked neuronal activity, providing exciting opportunities to assess network activity in association with feeding-related behavioral phenotypes.

This abstract and the presentation materials are available to members only; a login is required.

Join Here