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Abstract #2657

Pairwise metabolite-metabolite correlation analysis (MMCA) of HR-MAS 1H NMR spectra from 407 human brain tumours

Basetti Madhu1, Sean McGuire1, Alexandra Jauhiainen2, and John R Griffiths1

1Molecular Imaging (MRI & MRS), Cancer Research UK Cambridge Institute, Cambridge, United Kingdom, 2Early Clinical Biometrics, AstraZeneca AB R&D, Molndal, Sweden

Human brain tumour tissues from glioblastoma multiforme, astrocytoma, meningioma, oligodendroglioma and metastatic tumours were analyzed by metabolite-metabolite correlation analysis of HRMAS 1H NMR spectra from the eTumour database. The following metabolites were quantified using a modified LC-Model basis set: alanine (Ala), choline (Cho), creatine (Cr), lactate (Lac), glutamine (Gln), glutamate (Glu), glycine (Glyn), N-acetylaspartate (NAA), phosphocholine (PCh), phosphocreatine (PCr), taurine (tau), myo-inositol (Ino) and various lipids/macromolecules. The estimated metabolite concentrations from LCModel fittings were used in the investigation of pairwise metabolite-metabolite correlations. Pairwise metabolite-metabolite correlations can serve as an overview of metabolism and can be helpful in understanding the cellular metabolism.

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