Basetti Madhu1, Sean McGuire1, Alexandra Jauhiainen2, and John R Griffiths1
1Molecular Imaging (MRI & MRS), Cancer Research UK Cambridge Institute, Cambridge, United Kingdom, 2Early Clinical Biometrics, AstraZeneca AB R&D, Molndal, Sweden
Human
brain
tumour tissues from glioblastoma multiforme,
astrocytoma, meningioma,
oligodendroglioma and metastatic tumours
were analyzed
by metabolite-metabolite
correlation analysis
of HRMAS 1H NMR spectra from the eTumour database. The following
metabolites were quantified using a
modified LC-Model basis set: alanine (Ala), choline (Cho), creatine
(Cr), lactate
(Lac), glutamine (Gln), glutamate (Glu), glycine (Glyn), N-acetylaspartate
(NAA), phosphocholine (PCh), phosphocreatine
(PCr), taurine (tau), myo-inositol (Ino) and
various lipids/macromolecules. The
estimated metabolite
concentrations
from LCModel fittings were used in the investigation of
pairwise metabolite-metabolite correlations. Pairwise
metabolite-metabolite correlations can serve as an overview of
metabolism and
can be helpful in understanding the cellular metabolism.
