The motivation of this study was to investigate Magnetization Transfer MRI as a technique of contrast for visualization of the Purkinje Fiber (PF) network in ex vivo cardiac sample of a large animal. We first optimized MT parameters to obtain the best contrast: offset frequency, duration of the module and radiofrequency field. We performed 2D and 3D acquisition on pig ex vivo heart. We were able to enhance contrast between PF network and muscle and visualize insert points inside the myocardium in 3D.
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