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Abstract #3779

Mapping dynamics of epileptic seizures in GAERS using simultaneous BOLD fMRI and optical Ca2+ recordings

Lydia Wachsmuth1, Florian Schmid1, Franziska Albers1, Annika Lüttjohann2, Thomas Budde2, and Cornelius Faber1

1Department of Clinical Radiology, University of Münster, Münster, Germany, 2Institute of Physiology I, University of Münster, Münster, Germany

Simultaneous BOLD fMRI and optical Ca2+ recordings using the genetically encoded calcium indicator GCaMP were performed in a rat model of absence epilepsy (GAERS) for seizure mapping. Spike-and-wave discharge onset times and durations were derived from Ca2+ recordings and used for an event-related analysis with different hemodynamic response functions. BOLD maps showed large scale activations in cortical and subcortical areas, with delayed responses in subcortical areas. In contrast to electrophysiological recordings, Ca2+ recordings are not disturbed by MRI. They allow for cell-specific correlation with the hemodynamic response and provide a tool to obtain epileptic seizure maps with higher specificity.

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