Abstract #3779

Mapping dynamics of epileptic seizures in GAERS using simultaneous BOLD fMRI and optical Ca2+ recordings

Lydia Wachsmuth¹, Florian Schmid¹, Franziska Albers¹, Annika Lüttjohann², Thomas Budde², and Cornelius Faber¹

¹Department of Clinical Radiology, University of Münster, Münster, Germany, ²Institute of Physiology I, University of Münster, Münster, Germany

Simultaneous BOLD fMRI and optical Ca²⁺ recordings using the genetically encoded calcium indicator GCaMP were performed in a rat model of absence epilepsy (GAERS) for seizure mapping. Spike-and-wave discharge onset times and durations were derived from Ca²⁺ recordings and used for an event-related analysis with different hemodynamic response functions. BOLD maps showed large scale activations in cortical and subcortical areas, with delayed responses in subcortical areas. In contrast to electrophysiological recordings, Ca²⁺ recordings are not disturbed by MRI. They allow for cell-specific correlation with the hemodynamic response and provide a tool to obtain epileptic seizure maps with higher specificity.

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