The purpose of this study was to examine whether gagCEST imaging reflects the histopathological changes concerning glycosaminoglycan in human meniscus in vitro. All MRI acquisitions were performed on 7T MRI with a microimaging system. Histological staining using safranin-O was performed for correlation to imaging findings. Qualitatively, the gagCEST map and the corresponding safranin-O image show the same relative regional intensity of glycosaminoglycans. In sum, gagCEST imaging in a 7T microimaging system allows a very detailed look into the glycosaminoglycan distribution in the human meniscus.