We have performed a study of emotion regulation training in veterans with combat-related PTSD using real-time fMRI neurofeedback (rtfMRI-nf) with simultaneous EEG. Eighteen PTSD patients learned to upregulate their left amygdala activity using rtfMRI-nf during a positive emotion induction task based on retrieval of happy autobiographical memories. Enhancement in the amygdala-orbitofrontal functional connectivity during the rtfMRI-nf task showed positive correlation with severity of PTSD symptoms. Enhancement in left-lateralized upper alpha EEG coherence also positively correlated with PTSD severity. These results suggest that the rtfMRI-nf of the amygdala has the potential to correct the functional connectivity deficiencies specific to PTSD.