Several hypotheses associate the pathogenesis of discogenic low back pain with low pH. In this study, we used qCEST MRI technique to monitor the pH changes of the intervertebral disc in the process of degeneration in porcine model. A significant pH drop was observed at 2 weeks following degeneration induction. This trend is correlated with the expression of pain markers. These results suggest that qCEST MRI has the potential to serve as a novel and non-invasive method for the diagnosis of discogenic pain.